منابع مشابه
Biallelic Gene Targeting in Rice1[OPEN]
Plant Genome Engineering Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan (M.E., M.M., S.T.); Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa, 236-0027, Japan (M.M., S.T.); and Kihara Institute for Biological Research, Yokohama City University, 64...
متن کاملRunning head : Targeting of the Adh 2 gene in rice
The modification of an endogenous gene into a designed sequence by homologous recombination, termed gene targeting, has broad implications for basic and applied research. Rice (Oryza sativa L.), with a sequenced genome of 389 Mb, is one of the most important crops and a model plant for cereals, and the single-copy gene Waxy on chromosome 6 has been modified with a frequency of 1% per surviving ...
متن کاملPositive–negative-selection-mediated gene targeting in rice
Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding. However, in transformation of higher plants, non-homologous end joining (NHEJ) occurs overwhelmingly in somatic cells, masking HR-mediated GT. Positive-negative selection (PNS) is an approach f...
متن کاملSingle Gene in Rice'
A cDNA clone encoding rice (Oryza sativa L.) cytosolic triosephosphate isomerase (TPI), an important glycolytic enzyme, was isolated and characterized. l h e clone (pRTPI-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (M. 27,060). The identity of this clone was defined by its high homology (85% nucleotide sequence and 89% amino acid ...
متن کاملBiallelic and Genome Wide Association Mapping of Germanium Tolerant Loci in Rice (Oryza sativa L.)
Rice plants accumulate high concentrations of silicon. Silicon has been shown to be involved in plant growth, high yield, and mitigating biotic and abiotic stresses. However, it has been demonstrated that inorganic arsenic is taken up by rice through silicon transporters under anaerobic conditions, thus the ability to efficiently take up silicon may be considered either a positive or a negative...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2015
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.15.01663